The Effect Allium sativum (Garlic Extract) as Prebiotic Substance on the Activity of Probiotic Bacteria Lactobacillus acidophilus Against Some Locally Isolates of Pathogenic Bacteria

The aim of this study was to improve the growth and inhibitory activity selected Lactobacillus isolate.A total twenty samples dairy products (fresh yogurt) were collected. Seven isolates Lactobacillusspecies . obtained, identified as three fermentum, acidophilus andone brevis. lactobacillus counted , also ofLactobacillus against some pathogenic bacteria tested) for two different incubation periodsbefore addition Allium sativum(garlic extract) prebiotic substances. concentrationsof it used by toDe Man, Rogosa Sharpe agar (MRS) medium substances periods(24, 48) hour. effect sativum on probiotic evaluated, measurementof curve done after before adding garlic extract. For analyzinginhibitory with ,results showed that acidophiluspropagated in MRS fortified 20% 30% v/v extract period 24 hr giving thehighest inhibition zone.It concluded Lactobacillus.acidophilus isolate can grow well exhibit good Gramnegative a containing (that is added substrate supporting probioticgrowth activity). clear filtrate .acidophilus propagatingin spesific broth at 48 produce antibacterial effectbetter than 10% treatment enhance acidophilusespecially hr. may due fructooligosaccharide content when propagated MRSwithout treatment, which highly decrease pH cause growthof acidophillus.